Coding

Part:BBa_M11062

Designed by: Dan Nelson   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-13)


pfaC gene for omega-3 polyunsaturated fatty acid synthase

This is a single open reading frame which contains multiple genes that codes for necessary proteins in the sysnthesis of omega-3 polyunsaturated fatty acids. Ecosapentaenoic acid is the PUFA that is synthesized using this organism. The proteins coded within the gene are part of the families of Beta-ketoacyl synthase, Beta-hydroxyacyl-(acyl-carrier-protein) dehydratase- FabA/FabZ, Beta-ketoacyl synthase- N-terminal, and Beta-ketoacyl synthase- C-terminal. This gene sequence is to be used in sequence with pfaA, pfaB, and pfaD. By cloning these genes into the same organism, it should be able to produce omega-3 fatty acids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5147
    Illegal BglII site found at 5787
    Illegal BamHI site found at 4218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
    Illegal NgoMIV site found at 958
    Illegal NgoMIV site found at 1459
    Illegal NgoMIV site found at 1587
    Illegal NgoMIV site found at 2377
    Illegal NgoMIV site found at 3411
    Illegal AgeI site found at 520
    Illegal AgeI site found at 3027
    Illegal AgeI site found at 4912
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None